Essential oil content and composition of Achillea biebersteinii Afan. in different plant parts and phenological stages

Abstract

In this study, the essential oil content and composition of Achillea biebersteinii Afan. at different phenological stages including vegetative, floral budding, full flowering (leaves, stems and flowers) and fruit set were studied by means of gas chromatography (GC) and gas chromatography-mass spectometry (GC-MS). All oil samples from different plant parts and phenological stages were mostly made up of monoterpenoid compounds (88.6 - 99.6%), especially oxygenated ones (52.4 - 82.4%). The oil of the vegetative stage contained high amounts of limonene, 4aa-7β-7aa-nepetalactone, p-cymene and 1,8-cineole. The major constituents in the flower budding stage oil were found to be limonene, 1,8-cineole and 4aa-7β-7aa-nepetalactone. In the oil of the fruit set stage, c-terpinene, p-cymene and cis-chrysanthenyl acetate were the predominant constituents. On the other hand, the most important compounds from the stem oil were 4aa-7a-7aα-nepetalactone, 1,8-cineole, 4aa-7β-7aα-nepetalactone and camphor. 4a aα-7a-7a aα-nepetalactone, limonene, 1,8-cineole and cisp- menth-2-en-1-ol were found in high concentration in the oil of leaves, whereas 4aa-7a-7aα- nepetalactone, 4aα-7β- 7aα-nepetalactone, limonene and p-cymene were present in large amounts in the oil of flowers. © 2012 Taylor & Francis.