In vivo single cell labeling techniques

Abstract

Single cell labeling allows identification of neuron types based on neurite trajectories, an essential step to understand brain anatomy and function. For years, various techniques have been developed to achieve in vivo single cell labeling. In Drosophila, several genetic mosaic approaches have not only allowed labeling of specific neuron subsets but also enabled manipulations of gene function or neural activity in or outside the marked cell(s). They collectively provide an unprecedented level of versatility for diverse targeted studies. In this chapter, we selectively cover the general methods of single neuron labeling mediated by flip-out or mitotic recombination. © 2012 Springer Science+Business Media, LLC.