Study on the medicinal plants against grouper iridovirus infection: A short communication

Abstract

As one serious viral pathogen in mariculture, grouper Iridovirus (GIV) could cause high mortality to cultured groupers, then effective treatments against GIV infection are needed. The effects of Thlaspi arvense Linn. extracts against GIV infection was assessed by different methods in this study. The safe concentration of the water extract of Thlaspi arvense Linn. was initially determined as ≤1.25 mg/mL, which showed no obvious cytotoxic effects on cell viability. Using the safe concentration, aptamer(Q2)-based based fluorescent detection assay and qRT-PCR results indicated that, Thlaspi arvense Linn. water extracts had excellent inhibitory effects against GIV infection in vitro and in vivo. In addition, the mechanism of Thlaspi arvense Linn. on the invasion process of the grouper iridovirus was examined, which showed that Thlaspi arvense Linn. water extracts had significant inhibitory effects at the stage of virus adsorption on the host cell membrane. The inhibitory rate was 87.48%. Overall, Thlaspi arvense Linn. water extracts have great antiviral effects, making it an interesting candidate for developing effective medicines for fighting against GIV infection in farmed groupers.