Using hplc–dad and gc–ms analysis isolation and identification of anticandida compounds from gui zhen cao herbs (Genus bidens): An important chinese medicinal formulation

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Abstract

Gui Zhen Cao is an herbal formulation that has been documented in Chinese traditional medicine as a remedy for diarrhea, dysentery, inflammation, and toxicity. The sources of this formulation (Bidens pilosa L., Bidens biternata (Lour.) Merr. & Sherff, Bidens bipinnata L.) are also listed in ethnomedicinal reports all over the world. In this study, all these plants are tested for in vitro anticandida activity. A quantitative evaluation of the phytochemicals in all these plants indicated that their vegetative parts are rich in tannins, saponins, oxalates, cyanogenic glycoside and lipids; moreover, the roots have high percentages of alkaloids, flavonoids, and phenols. The results indicated significant anticandida activity, especially for the hexane extract of B. bipinnata leaves which inhibited C. albicans (42.54%), C. glabrata (46.98%), C. tropicalis (50.89%), C. krusei (40.56%), and C. orthopsilosis (50.24%). The extract was subjected to silica gel chromatography and 220 fractions were obtained. Purification by High Performance Liquid Chromatography with Diode-Array Detection (HPLC–DAD) and Gas Chromatography tandem Mass Spectrometry (GC-MS/MS) analysis led to the identification of two anticandida compounds: dehydroabietic and linoleic acid having an inhibition of 85 and 92%, respectively.

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Zahara, K., Bibi, Y., Masood, S., Nisa, S., Qayyum, A., Ishaque, M., … Chung, G. (2021). Using hplc–dad and gc–ms analysis isolation and identification of anticandida compounds from gui zhen cao herbs (Genus bidens): An important chinese medicinal formulation. Molecules, 26(19). https://doi.org/10.3390/molecules26195820

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