Hepatoprotective effect of cassia auriculata l. leaf extract on carbon tetrachloride intoxicated liver damage in wister albino rats

Abstract

Cassia auriculata B. belongs to Fabaceae and is widely distributed in India. It is used in traditional medicine, typically for skin disease, as a purgative, laxative, antihelminthic, antidiabetic and antioxidant from the ancient period. There have been reports of antidiabetic and medicinal properties of C. auriculata dried leaf and flowers. The present investigation was done to find whether the methanolic extract of Cassia auriculata leaf had hepatoprotective effect against carbon tetrachloride induced liver damage in Wister albino rats and to estimate the total antioxidant content, total phenolic content and total flavanoids of C. auriculata methanolic leaf extract. The methanol extract was used to treat the carbon tetra chloride induced liver damage on Wister albino rats for 60 days. In vitro antioxidant activity was studied using ABTS+ free radical scavenging method. The total content of phenolic compounds and flavanoids was also estimated by spectrophotometric method. The in vitro cytotoxicity activity was conducted on HepGinf2/inf cell line at increasing concentration and the apoptotic activity was determined. The animals were treated at 600 mg kg-1 b.wt. The blood serum was used for liver function test. Serum Bactate Dehydrogenase (BDH), Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Fyruvate Transaminase (SGPT) and γ-Glutamyl Transpeptidase (GGPT), Lipid Feroxidation (LPO) and liver tissue total protein were (p<0.001) significantly decreased in post treated animals. The non enzymatic antioxidant Reduced glutathione (GSH), Vitamin C (Vit C), Vitamin E (Vit E) and enzymatic antioxidant Glutathione Feroxidase (GPx), Glutathione-s-Transferase (GST), Superoxide Dismutase (SOD) and Catalase (CAT) were increased significantly in plant extract post treated group. The histopathological studies showed fine revertible changes in CAME treated experimental animal liver tissue section. © 2011 Academic Journals Inc.