Biological effect and multiple application potentials of carbon nanotubes have been becoming important issues attracting intense research interests and concerns. In this work, water dispersible oxidized multiwalled carbon nanotubes (o-MWNTs) were prepared by an optimal combination of ultrasonication and concentrated acids oxidation; stable concentrations of 0.3mg/ml were obtained in deionized water of surfactant free. The o-MWNTs dispersing in water displayed a strong characteristic adsorption at 253nm in the spectra of UV/VIS/NIR. XPS analysis showed the presence of carboxylated groups on the o-MWNTs. SEM observation indicated that after the oxidation, the as received MWNTs were cut short; the average length was around 500nm-800nm, the maximum effective size obtained by DLS measurement was about 340nm. It was inferred that the oxidation degree was one major key factor to determine the stable dispersion concentration of o-MWNTs in water. The o-MWNTs dispersing in water entered into the cells and located in the cytoplasm. Cells proliferation and living cycle were affected by the o-MWNTs dispersing in the culture medium in a concentration dependant manner; the cells cultivated in the medium with high concentration of o-MWNTs underwent apoptosis to a point determined by flowcytometry and TEM respectively, while low concentration of o-MWNTs induced light suppression of cells proliferation examined with cell viability assay. © 2008 Springer-Verlag.
CITATION STYLE
Meng, J., Yang, M., Song, L., Kong, H., Wang, C. Y., Wang, R., … Xu, H. Y. (2008). Preparation, characterization and cellular biological impact of water soluble multiwalled carbon nanotubes. In IFMBE Proceedings (Vol. 19 IFMBE, pp. 198–201). Springer Verlag. https://doi.org/10.1007/978-3-540-79039-6_51
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