Proceedings of the Frontiers in Retrovirology Conference 2018

Abstract

Oral presentationsSession 1: Entry & uncoatingO1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replicationIrena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh, Tobias Kern, Fabian Lindel, Gesche Gerresheim, Martin Hamann, Erik Müllers, Paul Lesbats, Peter Cherepanov, Erik Serrao, Alan Engelman, Dirk LindemannO2 A novel entry/uncoating assay reveals the presence of at least two species of viral capsids during synchronized HIV-1 infectionClaire Da Silva Santos, Kevin Tartour, Andrea CimarelliO3 Dynamics of nuclear envelope association and nuclear import of HIV-1 complexesRya Burdick, Jianbo Chen, Jaya Sastri, Wei-Shau Hu, Vinay PathakO4 Human papillomavirus protein E4 potently enhances the susceptibility to HIV infectionOliver T. KepplerSession 2: Reverse transcription & integrationO5 Structure and function of HIV-1 integrase post translational modificationsKarine Pradeau, Sylvia Eiler, Nicolas Levy, Sarah Lennon, Sarah Cianferani, Stéphane Emiliani, Marc RuffO6 Regulation of retroviral integration by RNA polymerase II associated factors and chromatin structureVincent ParissiSession 3: Transcription and latencyO7 A novel single-cell analysis pipeline to identify specific biomarkers of HIV permissivenessSylvie Rato, Antonio Rausell, Miguel Munoz, Amalio Telenti, Angela CiuffiO8 A capsid-dependent integration program linking T cell activation to HIV-1 gene expressionAlexander Zhyvoloup, Anat Melamed, Ian Anderson, Delphine Planas, Janos Kriston-Vizi, Robin Ketteler, Chen-Hsuin Lee, Andy Merritt, Petronela Ancuta, Charles Bangham, Ariberto FassatiO9 Characterisation of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genomeAnthony Rodari, Benoit Van Driessche, Mathilde Galais, Nadége Delacourt, Sylvain Fauquenoy, Caroline Vanhulle, Anna Kula, Arsène Burny, Olivier Rohr, Carine Van LintO10 Tissue-specific dendritic cells differentially modulate latent HIV-1 reservoirsThijs van Montfort, Renee van der Sluis, Dave Speijer, Ben BerkhoutSession 4: RNA trafficking & packagingO11 A novel cis-acting element affecting HIV replicationBo Meng, Andrzej Rutkowski, Neil Berry, Lars Dölken, Andrew LeverO12 Tolerance of HIV’s late gene expression towards stepwise codon adaptationThomas Schuster, Benedikt Asbach, Ralf WagnerSession 5: Assembly & releaseO13 Importance of the tax-inducible actin-bundling protein fascin for transmission of human T cell leukemia virus Type 1 (HTLV-1)Christine Gross, Veit Wiesmann, Martina Kalmer, Thomas Wittenberg, Jan Gettemans, Andrea K. Thoma-KressO14 Lentiviral nef proteins antagonize TIM-mediated inhibition of viral releaseMinghua Li, Eric O. Freed, Shan-Lu LiuSession 6: Pathogenesis & evolutionO15 SEVI and semen prolong the half-life of HIV-1Janis Müller, Jan MünchO16 CD169+ macrophages mediate retrovirus trans-infection of permissive lymphocytes to establish infection in vivoXaver Sewald, Pradeep Uchil, Mark Ladinsky, Jagadish Beloor, Ruoxi Pi, Christin Herrmann, Nasim Motamedi, Thomas Murooka, Michael Brehm, Dale Greiner, Thorsten Mempel, Pamela Bjorkman, Priti Kumar, Walther MothesO17 Efficient replication of a vpu containing SIVagm construct in African Green Monkeys requires an HIV-1 nef geneSimone Joas, Erica Parrish, Clement Wesley Gnanadurai, Edina Lump, Christina M. Stürzel, Nicholas F. Parrish, Ulrike Sauermann, Katharina Töpfer, Tina Schultheiss, Steven Bosinger, Guido Silvestri, Cristian Apetrei, Nicholas Huot, Michaela Müller-Trutwin, Daniel Sauter, Beatrice H. Hahn, Christiane Stahl-Hennig, Frank KirchhoffO18 Reprogramming initiates mobilization of endogenous mutagenic LINE-1, Alu and SVA retrotransposons in human induced pluripotent stem cells with consequences for host gene expressionGerald Schumann, Sabine Jung-Klawitter, Nina V. Fuchs, Kyle R. Upton, Martin Muñoz-Lopez, Ruchi Shukla, Jichang Wang, Marta Garcia-Canadas, Cesar Lopez-Ruiz, Daniel J. Gerhardt, Attila Sebe, Ivana Grabundzija, Patricia Gerdes, Sylvia Merkert, Andres Pulgarin, Anja Bock, Ulrike Held, Anett Witthuhn, Alexandra Haase, Ernst J. Wolvetang, Ulrich Martin, Zoltán Ivics, Zsuzsanna Izsvák, J. Garcia-Perez, Geoffrey J. FaulknerO19 NF-κB activation induces expression of human endogenous retrovirus and particle productionTara Hurst, Aris Katzourakis, Gkikas MagiorkinisSession 7a and b: Innate sensing & intrinsic immunityO20 Identification of the phosphatase acting on T592 in SAMHD1 during M/G1 transitionKerstin Schott, Rita Derua, Janna Seifried, Andreas Reuter, Heike Schmitz, Christiane Tondera, Alberto Brandariz-Nuñez, Felipe Diaz-Griffero, Veerle Janssens, Renate KönigO21 Vpx overcomes a SAMHD1-independent block to HIV reverse transcription that is specific to resting CD4 T cellsHanna-Mari Baldauf, Lena Stegmann, Sarah-Marie Schwarz, Maud Trotard, Margarethe Martin, Gina Lenzi, Manja Burggraf, Xiaoyu Pan, Oliver I. Fregoso, Efrem S. Lim, Libin Abraham, Elina Erikson, Laura Nguyen, Ina Ambiel, Frank Rutsch, Renate König, Baek Kim, Michael Emerman, Oliver T. Fackler, Oliver T. KepplerO22 The role of SAMHD1 in antiviral restriction and immune sensing in the mouseSabine Wittmann, Rayk Behrendt, Bianca Volkmann, Kristin Eissmann, Thomas GrambergO23 T cells expressing reduced restriction factors are preferentially infected in therapy naïve HIV-1 patientsSebastian Bolduan, Herwig Koppensteiner, Stefanie Regensburg, Ruth Brack-Werner, Rika Draenert, Michael SchindlerO24 cGAS-mediated innate immunity spreads through HIV-1 env-induced membrane fusion sites from infected to uninfected primary HIV-1 target cellsAurélie Ducroux, Shuting Xu, Aparna Ponnurangam, Sergej Franz, Angelina Malassa, Ellen Ewald, Christine GoffinetO25 Perturbation of innate RNA and DNA sensing by human T cell leukemia virus type 1 oncoproteinsSin-Yee Fung, Ching-Ping Chan, Chun-Kit Yuen, Kin-Hang Kok, Chin-Ping Chan, Dong-Yan JinO26 Induction and anti-viral activity of Interferon α subtypes in HIV-1 infectionUlf DittmerO27 Vpu-mediated counteraction of tetherin is a major determinant of HIV-1 interferon resistanceDorota Kmiec, Shilpa Iyer, Christina Stürzel, Daniel Sauter, Beatrice Hahn, Frank KirchhoffO28 DNA repair protein Rad18 restricts HIV-1 and LINE-1 life cycleYasuo Ariumi, Mariko Yasuda-Inoue, Koudai Kawano, Satoshi Tateishi, Priscilla TurelliO29 Natural mutations in IFITM3 allow escape from post-translational regulation and toggle antiviral specificityAlex Compton, Nicolas Roy, Françoise Porrot, Anne Billet, Nicoletta Casartelli, Jacob Yount, Chen Liang, Oliver SchwartzSession 8: Adaptive immunity & immune evasionO30 Observing evolution in HIV-1 infection: phylogenetics and mutant selection windows to infer the influence of the autologous antibody response on the viral quasispeciesCarsten Magnus, Lucia Reh, Penny Moore, Therese Uhr, Jacqueline Weber, Lynn Morris, Alexandra TrkolaO31 Dose and subtype specific analyses of the anti-HIV effects of IFN-alpha family membersRashel V. Grindberg, Erika Schlaepfer, Gideon Schreiber, Viviana Simon, Roberto F. SpeckSession 9: Novel antiviral strategiesO32 LEDGIN-mediated inhibition of the integrase-LEDGF/p75 interaction reduces reactivation of residual latent HIVZeger Debyser, Lenard Vranckx, Jonas Demeulemeester, Suha Saleh, Eric Verdin, Anna Cereseto, Frauke Christ, Rik GijsbersO33 NKG2D-mediated clearance of reactivated viral reservoirs by natural killer cellsO34 Inhibition of HIV reactivation in brain cells by AAV-mediated delivery of CRISPR/Cas9O35 CRISPR-Cas9 as antiviral: potent HIV-1 inhibition, but rapid virus escape and the subsequent design of escape-proof antiviral strategiesBen Berkhout, Gang Wang, Na Zhao, Atze T. DasSession 10: Recent advances in HIV vaccine developmentO36 Priming with a potent HIV-1 DNA vaccine frames the quality of T cell and antibody responses prior to a poxvirus and protein boostBenedikt Asbach, Josef Köstler, Beatriz Perdiguero, Mariano Esteban, Bertram L. Jacobs, David C. Montefiori, Celia C. LaBranche, Nicole L. Yates, Georgia D. Tomaras, Guido Ferrari, Kathryn E. Foulds, Mario Roederer, Gary Landucci, Donald N. Forthal, Michael S. Seaman, Natalie Hawkins, Steven G. Self, Sanjay Phogat, James Tartaglia, Susan W. Barnett, Brian Burke, Anthony D. Cristillo, Song Ding, Jonathan L. Heeney, Giuseppe Pantaleo, Ralf WagnerO37 Passive immunisation with a neutralising antibody against HIV-1 Env prevents infection of the first cells in a mucosal challenge rhesus monkey modelChristiane Stahl-Hennig, Viktoria Stab, Armin Ensser, Ulrike Sauermann, Bettina Tippler, Dennis Burton, Matthias Tenbusch, Klaus ÜberlaO38 HIV antibody Fc-glycoforms drive B cell affinity maturationGalit Alter, Giuseppe Lofano, Anne-Sophie Dugast, Viraj Kulkarni, Todd SuscovichPoster presentationsTopic 1: Entry & uncoatingP1 Dynein light chain is required for murine leukemia virus infectionTatiana Opazo, Felipe Barraza, Diego Herrera, Andrea Garces, Tomas Schwenke, Diego Tapia, Jorge Cancino, Gloria ArriagadaP2 Peptide paratope mimics of the broadly neutralising HIV-1 antibody b12Christina Haußner, Dominik Damm, Anette Rohrhofer, Barbara Schmidt, Jutta EichlerP3 Investigating cellular pathways involved in the transmission of HIV-1 between dendritic cells and T cells using RNAi screening techniquesRebecca Midgley, James Wheeldon, Vincent PiguetP4 Co-receptor tropism in HIV-1, HIV-2 monotypic and dual infectionsPriyanka Khopkar, Megha Rohamare, Smita KulkarniP5 Characterisation of the role of CIB1 and CIB2 as HIV-1 helper factorsAna Godinho-Santos, Allan Hance, Joao Goncalves, Fabrizio MammanoP6 Buffering deleterious polymorphisms in the highly constrained C2 region of HIV-1 envelope by the flexible V3 domainRomain Gasser, Meriem Hamoudi, Martina Pellicciotta, Zhicheng Zhou, Clara Visdeloup, Philippe Colin, Martine Braibant, Bernard Lagane, Matteo NegroniP7 Entry inhibition of HERV-K(HML-2) by an Env-IgG fusion proteinJula Wamara, Norbert BannertTopic 2: Reverse transcription & integrationP8 The R263K/H51Y re