Sphingomonas panacis sp. nov., isolated from rhizosphere of rusty ginseng

Abstract

The type strain DCY99 T was isolated from soil collected from a ginseng field in Hwacheon, Republic of Korea. Strain DCY99 T is Gram-negative, non-spore forming, motile, rod-shaped, and strictly aerobic. The bacteria grow optimally at 25–30 °C and pH 6.0–6.5. Phylogenetically, strain DCY99 T is most closely related to Sphingomonas oligophenolica JCM 12082 T , followed by Sphingomonas asaccharolytica KCTC 2825 T , Sphingomonas mali KCTC 2826 T , Sphingomonas cynarae JCM17498 T , Sphingomonas pruni KCTC 2824 T , and Sphingomonas glacialis DSM 22294 T . The DNA–DNA relatedness between strain DCY99 T and S. oligophenolica JCM 12082 T was 15.6 ± 0.4 %, and the DNA G+C content of strain DCY99 T was 64.4 mol%. An isoprenoid quinone was detected and identified as ubiquinone Q-10, and sym-homospermidine was identified as the major polyamine of DCY99 T . The major polar lipids were identified as sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylcholine. C 14:0 2OH, C 16:0 , and summed feature 8 (C 18:1 ω7c:/C 18:1 ω6c) were identified as the major fatty acids present in DCY99 T . The results of physiological and biochemical tests allowed strain DCY99 T to be differentiated phenotypically from other recognized species belonging to the genus Sphingomonas. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Sphingomonas panacis sp. nov. is proposed with the type strain designated as DCY99 T (=JCM 30806 T =KCTC 42347 T ).