Carbonic anhydrase i and II inhibition with natural products: Caffeine and piperine

Abstract

Novel chemotypes with carbonic anhydrase (CA; EC 4.2.1.1) inhibitory action, in addition to the sulphonamide and sulphamate were discovered, many of which are based on natural products. Caffeine and piperine were extracted and tested for inhibition of the human (h) cytosolic isoforms hCA I and II. The IC 50 values of caffeine against hCA I was of 55mM, whereas that of piperine of 60mM. The IC 50 values of caffeine and piperine against hCA II were of 2mM. Although these are quite weak inhibitors they may constitute leads for developing tighter binding compounds. © 2012 Informa UK, Ltd.