Abstract
The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.
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Kahler, A. M., & Hill, V. R. (2020). Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration. In Methods in Molecular Biology (Vol. 2052, pp. 23–41). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9748-0_3
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