96-Well DNA isolation method

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Abstract

The following protocol provides a high throughput, low cost method of producing a superior DNA yield of high quality which is suitable for TILLING, map based cloning or any application which requires long term DNA storage. The protocol has been designed for DNA extraction from leaf material, preferably young leaf tissue should be used as this minimises samples being contaminated with polysaccharides and phenolics.

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Perry, J., & Parniske, M. (2005). 96-Well DNA isolation method. In Lotus Japonicus Handbook (pp. 129–131). Springer Netherlands. https://doi.org/10.1007/1-4020-3735-X_11

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