Mitophagy is a cellular process of autophagy-based mitochondrial degradation that eliminates dysfunctional mitochondria and ensures cellular homeostasis. In pancreatic islet β-cells, mitochondria play a pivotal role in glucose-stimulated insulin secretion through ATP production from glucose oxidation. Recent studies have shown that impaired mitophagy and the subsequent mitochondrial compromise contribute to β-cell dysfunction and glucose intolerance. In this chapter, we describe a protocol to monitor Parkin-mediated mitophagy in pancreatic MIN6 β-cells using flow cytometry and a pH-sensitive fluorophore, mKeima.
CITATION STYLE
Hoshino, A., & Matoba, S. (2018). Observation of Parkin-mediated mitophagy in pancreatic β-cells. In Methods in Molecular Biology (Vol. 1759, pp. 41–46). Humana Press Inc. https://doi.org/10.1007/7651_2017_39
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