Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)

Abstract

Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP -8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP -8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP -8 in GCF.