Purpose: Identification of human insulin analogs’ impurity with a mass shift +14 Da in comparison to a parent protein. Methods: The protein sequence variant was detected and identified with the application of peptide mapping, liquid chromatography, tandem mass spectrometric analysis, nuclear magnetic resonance spectroscopy (NMR) and Edman sequencing. Results: The misincorporated lysine (Lys) at asparagine (Asn) position A21 was detected in recombinant human insulin and its analogs. Conclusions: Although there are three asparagine residues in the insulin derivative, the misincorporation of lysine occurred only at position A21. The process involves G/U or A/U wobble base pairing.
CITATION STYLE
Stadnik, D., Bierczyńska-Krzysik, A., Zielińska, J., Antosik, J., Borowicz, P., Bednarek, E., … Kozerski, L. (2019). Identification of Lysine Misincorporation at Asparagine Position in Recombinant Insulin Analogs Produced in E. coli. Pharmaceutical Research, 36(6). https://doi.org/10.1007/s11095-019-2601-z
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