Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509

Abstract

Enteropathogenic Escherichia coli (EPEC) expressing the Type III secretion system (TTSS) induced hemolysis of sheep blood cells. Using this assay, six structurally related compounds designated as guadinomines were isolated as inhibitors of TTSS-induced hemolysis by ion exchange column chromatography and HPLC from the culture broth of Streptomyces sp. K01-0509. Guadinomines A and B showed potent inhibition with IC50 values of 0.02 and 0.007 μg/ml, respectively, guadinomine D showed moderate activity (IC50: 8.5 μg/ml), while guadinomines C1 and C2 and guadinomic acid had no activity.