Isolation of Coxsackie A type 16 and other types of Coxsackie A group viruses from hand, foot and mouth disease patients in Hokkaido in summer, 1970 (Japanese)

Abstract

An epidemic of hand, foot and mouth disease occurred in Hokkaido in the summer of 1970. From 153 patients, 74 fecal samples, 124 throat swabs, and a sample of vesicular fluid were collected. A total of 79 Cox A 16 viruses and 25 unidentified viruses were isolated from them by inoculation into primary monkey kidney cells. In addition 6l Cox A 16, 17 Cox A4, 27 Cox A5, 63 Cox A6, and 9 Cox A10 viruses were isolated from them by inoculation into suckling mice. Of 45 patients from whom both fecal and throat samples had been collected, Cox A16 was isolated singly from 7 patients, Cox A16 and some other types were in combination from 34 patients, and Cox A viruses other than Cox A16 were from 3 patients. Cox A5 (from 2 patients), Cox A6 (from 3 patients), Cox A10 (from 2 patients) were regarded as causative agents in addition to Cox A16. Arise of antibody against each of these viruses was as follows: Cox A16 (16.7%), Cox A5 (16,7%), Cox A6 (11.4%), Cox A2 (2.8%), Cox A4 (2.8%), Cox A10 (2.8%) when examined in 42 paired sera (of which 7 paired sera were tested only for Cox A16 and Cox A5). A simultaneous rise of antibody against 2 types was seen in 4 paired sera. From virological and serological findings, it was suggested that Cox A5, Cox A6, and Cox A10 viruses might be able to play a role as causative agents in an outbreak of hand, foot and mouth disease.