The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires' disease is likely due to the high number of virulence-related proteins in the vesicles. OMVs are isolated from the supernatant of liquid cultures of L. pneumophila. After low-speed centrifugation, residual bacteria and cell fragments are eliminated by passing the supernatant through a filter. OMVs are pelleted by ultracentrifugation and resuspended in buffer. The isolated OMVs can be analyzed for their molecular components and their interactions with host structures, bacterial cells, or surfaces. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Jäger, J., & Steinert, M. (2013). Enrichment of outer membrane vesicles shed by Legionella pneumophila. Methods in Molecular Biology, 954, 225–230. https://doi.org/10.1007/978-1-62703-161-5_13
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