Estimation of Total Phenol Content and Antimicrobial Activity in Different Leaf Stage of Lepisanthes amonea

Abstract

Many plants have been reported to possess antibacterial activity, and among them are coming from the Sapindaceae family. The Sapindaceae family has 2,215 species in 147 genera. L. amoena is one species in this family found in East Kalimantan. This study's objective was to determine the effect of the leaf stage on phenolic composition and antimicrobial activity. The powder of L. amoena was extracted by successive maceration using hexane, ethyl acetate, and ethanol. In this study, the polyphenolic content of the different extracts was determined by the Folin-Ciocalteu method. Their antimicrobial activity was assayed through in vitro models such as agar dilution assay. The anti microorganism of different leaf stage from L. amoena extracts towards Streptococcus mutans, Propionibacterium acnes, and Candida albicans growth were determined. The results showed that Lepisanthes amoena extracts could inhibit the growth of those bacteria. The expression level of inhibitory effect on bacteria's growth was reduced by increasing the concentration of extract. The leaves at a particular stage may have more antimicrobial potential. The leaf maturity played essential roles in the bioactive compounds and their antimicrobial of L. amoena leaf extract.