A water relations analysis of seed germination rates

Abstract

Seed germination culminates in the initiation of embryo growth and the resumption of water uptake after imbibition. Previous applications of cell growth models to describe seed germination have focused on the inhibition of radicle growth rates at reduced water potential (ψ). An alternative approach is presented, based upon the timing of radicle emergence, to characterize the relationship of seed germination rates to ψ. Using only three parameters, a 'hydrotime constant' and the mean and standard deviation in minimum or base ψ among seeds in the population, germination time courses can be predicted at any ψ, or normalized to a common time scale equal to that of seeds germinating in water. The rate of germination of lettuce (Lactuca sativa L. cv Empire) seeds, either intact or with the endosperm envelope cut, increased linearly with embryo turgor. The endosperm presented little physical resistance to radicle growth at the time of radicle emergence, but its presence markedly delayed germination. The length of the lag period after imbibition before radicle emergence is related to the time required for weakening of the endosperm, and not to the generation of additional turgor in the embryo. The rate of endosperm weakening is sensitive to ψ or turgor.