We studied the role of charge recombination reactions in Photosystem II photodamage in Synechocystis 6803 cells, in which the redox properties of Phe were modified. In the Q130E mutant where Em(Phe/Phe−) is shifted by about +35 mV photodamage and 1O2 production is decreased relative to the WT, whereas the opposite effect was observed in the Q130L mutant where Em(Phe/Phe−) is shifted by about −50 mV. We conclude that the extent of photodamage is influenced by competition of dissipation pathways of 1[P680 +Phe−] via non-radiative charge recombination and spin conversion to 3[P680 +Phe−], which regulates the yield of 3P680 and related 1O2 production. The non-radiative recombination rate of 1[P680+Phe−] is affected by Em(Phe/Phe−) whose modulation by the D1-Q130E amino acid replacement appears to be a photoprotective mechanism in cyanobacteria in vivo.
CITATION STYLE
Burda, K., Schöttler, M. A., Orzechowska, A., Kruk, J., Strzałka, K., & Bock, R. (2008). Regulatory Function of Cytochrome b559 in Photosystem II. In Photosynthesis. Energy from the Sun (pp. 563–567). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6709-9_127
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