Marka Mikrosatelit sebagai Alternatif Uji BUSS dalam Perlindungan Varietas Tanamam Padi

Abstract

This study was conducted at the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development to seek the feasibility of using SSR markers for DUS testing in rice variety. Released variety Fatmawati together with seven other reference varieties were analyzed using genetic analyzer for their variability, ten SSR markers were used. Hundred and twenty five alleles located on chromosomes 1, 2, 5, 6, 7, 10, and 11 were detected in eight varieties studied, where the number of alleles ranging from 6 (BPC) to 33 (Fatmawati), with the predominant markers such as RM11 on Fatmawati, Maros, Barumun, Gilirang, and Memberamo varieties, RM237 on Cisadane and BP630 varieties, and RM133 and RM287 markers on BPC variety. The genetic distance-based results in the unrooted neighbor-joining tree revealed that the eight varieties as well as their populations clustered separately. Genetic variability within each variety indicated that these varieties were still in higher degree of heterogeneity. This demonstrated the power of SSR marker in differentiating varieties as well as population within variety, which, therefore, SSR marker could be recommended in plant variety protection.