Isolation and characterization of volatile oil by GC-MS analysis and screening of in vitro antioxidant potential of hydroalcoholic extracts of some indigenous plants of northern India in rodents

Abstract

The main aim and objective of my research work is the isolation of volatile oil from the leaves of plants by GC-MS analysis and screening of in vitro antioxidant potential of hydroalcoholic extracts of some Indigenous plants of Northern India in rodents. To perform this research work the fresh leaves of Prunus persica, Calotropis procera & Canscora decussata were taken. The isolation of volatile oil was done by using Clavenger apparatus. The samples were further analyzed by Gas Chromatography-Mass Spectrometry. In vitro antioxidant potential was carried out by DPPH, Hydrogen peroxide, Hydroxyl radical and Nitric oxide scavenging activity. Ascorbic acid was used as standard drug in this study. The percent inhibition values for antioxidant potential were obtained and tabulated. The present experimental data clearly displayed that the hydroalcoholic extract of above mentioned plant's leaves exhibited antioxidant potential.