Adeno-associated virus (AAV) is one of the most promising vectors for gene therapy. There are several ways of producing AAV vectors but large-scale production of this vector remains a major challenge. Virovek developed a novel method of expressing the AAV Rep and Cap genes in insect cells mediated by intron-splicing mechanism and producing AAV vectors with these Rep and Cap sequences containing the artificial intron. The recombinant baculoviruses harboring these artificial intron-containing Rep and Cap sequences are very stable and the AAV vectors produced in insect cells with these recombinant baculoviruses are very infectious.
CITATION STYLE
Chen, H. (2011). Manufacturing of Adeno-Associated Viruses, for Example: AAV2. In Methods in Molecular Biology (Vol. 737, pp. 235–246). Humana Press Inc. https://doi.org/10.1007/978-1-61779-095-9_10
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