Inhibition of erythrocyte hemolysis induced by H2O2 with Mangifera indica L. Extract

Abstract

Oxidative stress is originated by a high production of ROS (pro-oxidant process), along with a impaired antioxidant mechanisms in the organism, which leads to the damage of different macromolecules. One of the most important targets of ROS are the polyunsaturated fatty acids (PUFA) of cell membranes. These damages produce changes in the structure and function of the cells, favoring process like premature aging and the appearance of different degenerative and autoimmune diseases. In this study it was evaluated the effect of Mangifera indica L. extracts treatments over the Human Erythrocyte Hemolysis caused by the pro-oxidant agent H2O2. On the other hand, in order to analyze cell membrane changes induced by the treatments, erythrocytes sediments were observed and photographed with an inverted microscope, using the phase contrast tool. Erythrocytes were obtained from healthy donors, and the test samples were prepared with 250 μl of erythrocyte suspension, 250 μl of H2O2. (1000mM or 100mM) and 1000 μl of mango extracts (30, 50, 100, 150, 200μg/ml); curcuma extracts at 50 μg/ml were used as control. Percentages of hemolysis inhibition over 49% were observed with mango treatments of 30 and 50 μg/ml. Likewise, the mango treatments shown a protective activity, which was evidenced by the conservation of the erythrocyte membranes treated with H2O2. Our results suggest that the damages suffered by the erythrocyte membrane by the oxidant action of H2O2 can be reduced by the action of compounds that are present in the Mango extracts.