Interleukin 17 (IL-17) increases the expression of toll-like receptor-2, 4, and 9 by increasing IL-1β and IL-6 production in autoimmune arthritis

32Citations
Citations of this article
27Readers
Mendeley users who have this article in their library.

Abstract

Objective. To examine the effect of interleukin 17 (IL-17) on the expression of Toll-like receptor (TLR)-2, 4, and 9 in collagen-induced arthritis (CIA) in mice. Methods. On Days 28 and 32 after induction of CIA in mice, phosphate-buffered saline (PBS group) or IL-17 (IL-17 group) was injected into both knee joints. On Day 35, mice were sacrificed. The severity of knee joint arthritis, synovial inflammation, and bone destruction was measured by a scoring system using macrography and histological analysis. Synovial expression of TLR-2, 4, 9, IL-17, IL-1β, tumor necrosis factor-α (TNF-α), and IL-6 was determined by real-time PCR and immunohistochemistry. Synoviocytes of CIA mice were cultured with IL-17 and with neutralizing antibodies to cytokine, and the expression of TLR-2, 4, 9, IL-1β, TNF-α, and IL-6 was determined by real-time RT-PCR. Results. In CIA mice, knee arthritis scores, synovial inflammation, bone destruction scores, and expression of synovial TLR-2, 4, and 9, IL-17, IL-1β, TNF-α and IL-6 were higher in the IL-17 and PBS groups than in normal DBA1 mice. These variables were also significantly higher in the IL-17 group than in the PBS group. In CIA synoviocytes, IL-17 increased the expression of TLR-2, 4, and 9, and this effect was significantly alleviated by neutralizing antibodies to IL-17, IL-1β, and IL-6. Conclusion. IL-17 aggravates joint inflammation and destruction, and increases the synovial expression of TLR-2, 4, and 9 by increasing IL-1β and IL-6. These results imply that the IL-17-induced increase in expression of TLR-2, 4, and 9, and IL-1β and IL-6 production are involved in the IL-17-induced aggravation of arthritis. The Journal of Rheumatology Copyright © 2009. All rights reserved.

Cite

CITATION STYLE

APA

Lee, J. H., Cho, M. L., Kim, J. I., Moon, Y. M., Oh, H. J., Kim, G. T., … Kim, S. I. (2009). Interleukin 17 (IL-17) increases the expression of toll-like receptor-2, 4, and 9 by increasing IL-1β and IL-6 production in autoimmune arthritis. Journal of Rheumatology, 36(4), 684–692. https://doi.org/10.3899/jrheum.080169

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free