Background: Polymerase chain reaction (PCR) analysis is an important tool in the diagnosis of infectious uveitis. A retrospective, interventional study of PCR analysis of ocular fluid in suspected infectious uveitis cases between January 2014 to July 2016 was done. Nested, real-time and broad range PCR was performed for detection of the genome of Mycobacterium tuberculosis, herpes virus family, Chikungunya virus, Toxoplasma gondii, fungus, eubacterium and propionibacterium acne. Results: Total of 100 cases included, mean age was 39.2 ± 15.4 years. Uveitis was unilateral in 82% and granulomatous in 40%. Mean visual acuity at the initial visit and final visit was 0.73 logMar and 0.63 logMar respectively. PCR analysis confirmed the clinical diagnosis in 70.1% patients. The sensitivity, specificity, positive predictive value and negative predictive value of PCR analysis was 90.2%, 93.9%, 93.9% and 90.2% respectively. The quantitative value of real-time M. tb. Positive PCR ranged from 32c/ml to 2722 c/ml. Conclusions: PCR assay is an accurate technique with high sensitivity and specificity to diagnose the DNA genome in infectious uveitis.
CITATION STYLE
Kharel (Sitaula), R., Janani, M. K., Madhavan, H. N., & Biswas, J. (2018). Outcome of polymerase chain reaction (PCR) analysis in 100 suspected cases of infectious uveitis. Journal of Ophthalmic Inflammation and Infection, 8(1). https://doi.org/10.1186/s12348-017-0144-1
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