We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477–89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.
CITATION STYLE
Tang, Q., Gernoux, G., Cheng, Y., Flotte, T., & Mueller, C. (2020). Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. In Methods in Molecular Biology (Vol. 2164, pp. 75–85). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0704-6_9
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