Reconstruction of full-length isoforms from splice graphs

Abstract

Most alternative splicing events in human and other eukaryotic genomes are detected using sequence fragments produced by high throughput genomic technologies, such as EST sequencing and oligonu-cleotide microarrays. Reconstructing full-length transcript isoforms from such sequence fragments is a major interest and challenge for computational analyses of pre-mRNA alternative splicing. This chapter describes a general graph-based approach for computational inference of full-length isoforms. © 2008 Humana Press, a part of Springer Science+Business Media, LLC.