The Clostridium botulinum GerAB germination protein is located in the inner membrane of spores

14Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

Clostridium botulinum dormant spores germinate in presence of l-alanine via a specific receptor composed of GerAA, GerAB and GerAC proteins. In Bacillus subtilis spores, GerAA and GerAC proteins were located in the inner membrane of the spore. We studied the location of the GerAB protein in C. botulinum spore fractions by Western-blot analysis, using an antipeptidic antibody. The protein GerAB was in vitro translated and used to confirm the specificity of the antibodies. GerAB was not present in a coat and spore outer membrane fraction but was present in a fraction of decoated spores containing inner membrane. These results strongly suggest that the protein GerAB is located in the inner membrane of the spore. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Alberto, F., Botella, L., Carlin, F., Nguyen-the, C., & Broussolle, V. (2005). The Clostridium botulinum GerAB germination protein is located in the inner membrane of spores. FEMS Microbiology Letters, 253(2), 231–235. https://doi.org/10.1016/j.femsle.2005.09.037

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free