Evaluation study of Matricaria chamomilla extract toward pathogenic isolates of Candida

Abstract

Candidiasis is the most common type of yeast infection; the causative agent of this infection is Candida spp. Mostally candida albicans which normally lives on the skin and inside the body cavities such as the mouth, throat, gut, and vagina, without causing infection. Candida can cause infections if it grows out of control especially in immunocompromised Patients or if it enters deep into the body for example, the bloodstream or internal organs. Candidiasis can be treated either by topicals or systemic antifungal but some time these drugs are useless because some yeasts aquarid resistance to it also the antifungals have many side effects due to similarity between fungal and human cells so that both eukaryotic cells. This study was designed to isolate and identify pathogenic yeasts that cause oral and vaginal candidiasis. To achieve these goal, 20 swab samples positive were obtained from patients and cultured and identified morphologically and by molecular methods. Fourteen biologically active chemical compounds were identified by GC-MS analysis in a methanol crude extracted from the flowers of the Matricaria chamomilla plant. The cytotoxicity assay of M. chamomile methanol extract was showed there is no cytotoxic affecting human solution. This extract has a high inhibitory capacity toward Candida antifungal (fluconazole, nystatin) showed an inhibitory effect on Candida and some C. albicans showed resistance to these antifungal used.