Raw sequence data and quality control

Abstract

Next-generation sequencing technologies are extensively used in many fields of biology. One of the problems, related to the utilization of this kind of data, is the analysis of raw sequence quality and removal (trimming) of low-quality segments while retaining sufficient information for subsequent analyses. Here, we present a series of methods useful for converting and for refinishing one or more sequence files. One of the methods proposed, based on dynamic trimming, as implemented in the software StreamingTrim allows a fast and accurate trimming of sequence files, with low memory requirement.