Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.
CITATION STYLE
Karlsson, C., Teleman, J., & Malmström, J. (2017). Analysis of bacterial surface interactions with mass spectrometry-based proteomics. In Methods in Molecular Biology (Vol. 1535, pp. 17–24). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6673-8_2
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