Aptamers are oligonucleotides displaying specific binding properties for a predetermined target. They can be easily immobilized on various surfaces such as nanoparticles. Functionalized particles can then be used to various aims. We took advantage of the AlphaScreen® technology for monitoring aptamer-mediated interactions. A particle bearing an aptamer contains a photosensitizer whereas another type of particle contains a chemiluminescer. Irradiation causes the formation of singlet oxygen species in the photosensitizercontaining bead that in turn activates the chemiluminescer. Luminescence emission can be observed if the two types of beads are in close proximity (<200 nm). This is achieved when the cognate ligand of the aptamer is grafted onto the chemiluminescer-containing bead. Using this technology we have screened oligonucleotide libraries and monitored aptamer–protein interactions. This constitutes the basis for aptamer-based analytical assays.
CITATION STYLE
Evadé, L., Dausse, E., Taouji, S., Daguerre, E., Chevet, E., & Toulmé, J. J. (2015). Aptamer-mediated nanoparticle interactions: From oligonucleotide–protein complexes to SELEX screens. Methods in Molecular Biology, 1297, 153–167. https://doi.org/10.1007/978-1-4939-2562-9_11
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