Construction of expression vector of miRNA specific for FUT3 and identification of its efficiency in KATO-III gastric cancer cell line

Abstract

Human blood group Lewis antigen, which is fucosylated glycoconju-gates involved in the development of several pathologies. Fucosyltransferases are enzymes that add fucose to precursor glycan structures: FUT3 catalyze the addition of fucose to the a 1-3, 4 position and are detected in epithelial cells. As the expression of Lewis a is mainly controlled by FUT3, which can influence Lewis a'S synthesis, we choose FUT3 as our target gene to silence. In our study, we constructed FUT3-specific miRNA expression vector successfully. It showed in the experiment that FUT3 - specific miRNA expression vector could actively inhibit FUT3′S expression in mRNA level in KATO - III gastric cancer cel1 line, and decrease Lewis antigen'S synthesis as welI growth. From this experiment, we obtain the best mi RNA sequence of FUT3, which may serve as a new strategy for investigating the mechanism of molecular glycol - pathology of gastric cancer targeted tumor gene therapy. © Springer Science+Business Media Dordrecht 2014.