Abstract
A gas syringe method was used to evaluate the effect of secondarycompounds from plant materials on in vitro fermentation products andmicrobial biomass. The experiment used Pennisetum purpureum, Morindacitrifolia fruit, Nothopanax scutellarium leaves, Sesbania seshan LS(low saponins type), Sesbania sesban HS (high saponins type) andSapindus rarak fruit as substrates. The incubation was conducted withand without polyethylene glycol 6000 (PEG) addition for 24 hours. Gasproduction and short-chain fatty acids (SCFA) were analysed. Prokaryoticand eukaryotic concentrations were measured by quantifying 16S and 18SrRNA.The percentage increase in gas production due to PEG was very small (
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CITATION STYLE
Wina, E., Muetzel, S., Hoffman, E., Makkar, H. P. S., & Becker, K. (2005). Effect of Secondary Compounds in Forages on Rumen Micro-organisms Quantified by 16S And 18S rRNA. In Applications of Gene-Based Technologies for Improving Animal Production and Health in Developing Countries (pp. 397–410). Springer-Verlag. https://doi.org/10.1007/1-4020-3312-5_30
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