The use of fish scale epithelia in non-destructive methods of karyotyping Oreochromis niloticus L. and Cyprinus carpio L. for experimental gynogenetic studies

Abstract

Metaphase figures suitable for karyological investigations in fish were produced by the use of non-invasive and non-destructive methodologies. Large numbers of dividing scale epithelial cells were obtained by two methods: First, scale epithelia of Oreochromis niloticus were cultured for 22 hours at 28°C, and second, scale epithelia of Cyprinus carpio were used without culturing after the addition of a metaphase inhibitor. These techniques gave typical karyotypes in control carp and tilapia and confirmed absence of paternal (carp) chromosomes in gynogenetic tilapias. © 1994 Taylor & Francis Group, LLC.