Application of bioactive coatings based on chitosan and propolis for Pinus spp. Protection against Fusarium circinatum

Abstract

Pine pitch canker (PPC) is a major threat to pine forests worldwide because of the extensive tree deaths, reduced growth, and degradation of timber quality caused by it. Furthermore, the aggressive fungus responsible for this disease (Fusarium circinatum) can also infect pine seeds, causing damping-off in young seedlings. This study proposes an approach based on coating treatments consisting of natural products to ensure seed protection. Seeds from two pine species (the most sensitive to this disease, Pinus radiata D. Don, and a more resistant one, Pinus sylvestris L.) were coated with single and binary mixtures of low and medium molecular weight chitosan and/or ethanolic-propolis extract. The germination rate, pre- and post-emergence mortality, total phenolic content, and radical scavenging activity were assessed. All treatments, and especially the one based on chitosan oligomers, had a beneficial impact on P. sylvestris seedlings, significantly enhancing survival rates and displaying a positive influence on the total phenolic content and on the seedlings' radical scavenging activity. Conversely, non-significant negative effects on germination percentages were observed in the case of P. radiata seeds. The proposed treatments show promise for the protection of P. sylvestris seedlings against PPC.