Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay

Abstract

Padlock probes and rolling circle amplification are techniques which can be used for detection of DNA sequences in situ with high specificity and high signal to noise. The single-cell gel electrophoresis assay is used to measure DNA damage and repair in cells. Here, we describe how padlock probes and rolling circle amplification can be used to detect DNA sequences within comet preparations. © 2012 Springer Science+Business Media, LLC.