Modelling citrate metabolism in fruits: Responses to growth and temperature

Abstract

Citrate production and degradation during the last stage of fruit development were modelled by representing the fluxes through the enzymes of the citrate cycle and the malic enzyme, the transport of metabolites between the cytosol and the mitochondria, and the stoichiometry equations that relate these reactions. After solving the corresponding system of equations, the rate of citrate synthesis (or degradation) was expressed as a simple function of temperature, mesocarp weight, and respiration. The model was applied to peach fruit, and its parameters were estimated from the data of a 2-year field experiment. The predictions of the model were in agreement with experimental data. Simulations were made to analyse the responses to variations of temperature and fruit growth. Increasing fruit growth before stone hardening stimulated citrate production, while increasing fruit growth after stone hardening reduced it. Delaying the date at which the maximum growth rate was reached enhanced citrate production during most of the period. In the last weeks before harvest, increasing temperature depressed citrate production, while, at the beginning of the period studied, it enhanced it.