Optimasi ekstrak kulit ranting sengon terhadap bakteri Pseudomonas sp, Escherichia coli, Staphylococcus aureus, dan Proteus Sp

Abstract

Excessive use of antibiotics can lead to bacterial resistance, therefore the use of natural ingredients is expected to reduce these negative effects. One plant that has the potential for antibiotics is sengon. Increased consumption of sengon as a household need causes the waste produced to increase every year. This study aims to determine the effectiveness and optimal concentration of sengon twig bark extract with solvent N-hexane, methanol, ethyl acetate in Pseudomonas Sp, Escherichia coli, Staphylococcus aureus, Proteus sp by using in vitro method. The in vitro method used in this study was Kirby bauer. The parameters observed were the formed zone of inhibition, namely by measuring the diameter of the clear zone around the disc paper with a ruler. The concentrations used in this study were 9%, 9.5%, 10%, 10.5%, and 11%. The five concentration levels of the sample are potential to inhibit the bacteria Pseudomonas sp, Escherichia coli, Staphylococcus aureus, Proteus sp. The test results conducted showed that the sengon twig bark extract dissolved with several types of solvents had antibacterial activity. The largest zone of inhibition was shown at a concentration of 10.5% of sengon twig bark extract with methanol solvent against Escherichia coli bacteria with a diameter of 10 mm. It was concluded that the sengon twig bark extract could effectively inhibit the growth of the bacteria Pseudomonas sp, Escherichia coli, Staphylococcus aureus, Proteus sp.