Lanostane triterpenes from the fungus Pisolithus tinctorius

Abstract

A new triterpene, 3β,22ξ,23ξ-trihydroxy-24-methyllanosta-8, 24(28)-diene-31-a1 22-acetate, named pisosteral, and four known lanostane triterpenes derivatives, 3β,22ξ,23ξ-trihydroxy-24-methyllanosta-8, 24(28)-diene 22-acetate (pisosterol), 3α,22ξ,23ξ-trihydroxy-24- methyllanosta-8,24(28)-diene 22-acetate (3-epi-pisosterol), 3β,22ξ, 23ξ-trihydroxy-24-methyllanosta-8,24(28)-diene 23-acetate and 3β,22ξ,23ξ-trihydroxy-24-ethyllanosta-8,24(28)-diene 22-acetate, were isolated from fruiting bodies of Pisolithus tinctorius, collected in an Eucalyptus plantation. From cultivation of a strain of P. tinctorius isolated from Pinus taeda, four known triterpenes lanostane derivatives, 3β,22ξ-dihydroxy-24-methyllanosta-8,24(28)-diene,3β, 22ξ-dihydroxy-24-ethyllanosta-8,24(28)-diene, 3β-hydroxylanosta-8,24- diene (lanosterol) and 3β-hydroxylanosta-7(8),9,24-triene (agnosterol), were isolated. These compounds were identified on the basis of chemical and physical methods, mainly mass spectrometry and ID and 2D nuclear magnetic resonance. ©2005 Sociedade Brasileira de Química.