Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene

Abstract

We have previously described the elements of two overlapping promoters regulating differential expression of the DictyosteliumP8A7 gene. One promoter was constitutively active but stimulated during development (S-promoter), while the other (L-promoter) was found to be induced by a variety of stimuli. This effect was mediated by the induction (i-) element. By a series of antisense transformation experiments we here provide evidence that this element is the target of a feedback control circuit. In wild type cells, feedback regulation activates the L-promoter in unfavourable environmental situations when transcription from the S-promoter is insufficient to generate adequate amounts of protein. A model for the function of the feedback loop under natural and experimental conditions is presented. © 1990 Oxford University Press.