We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, effectively cleaves double-stranded DNA, and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues. © 2005 Pleiades Publishing, Inc.
CITATION STYLE
Zhulidov, P. A., Bogdanova, E. A., Shcheglov, A. S., Shagina, I. A., Wagner, L. L., Khazpekov, G. L., … Shagin, D. A. (2005). A method for the preparation of normalized cDNA libraries enriched with full-length sequences. Russian Journal of Bioorganic Chemistry, 31(2), 170–177. https://doi.org/10.1007/s11171-005-0023-7
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