Enhancement of antibacterial activity of suede leather through coating silver nanoparticles synthesized using piper betle

Abstract

The objective of this study was to modify suede leather by coating Ag nanoparticles which synthesized with an extract from green betel leaf. The success of suede leather modification with silver nanoparticles was analyzed by FTIR spectrophotometer, SEM tool, and antibacterial activity test. Characteristic of Ag nanoparticles was analyzed through using a UV-Vis spectrophotometer. The stages of experimental research carried out where the extraction of betel leaves, preparation of Ag nanoparticles with betel leaf extract using precursors of silver nitrate solution, characterization of Ag nanoparticles with Ultra Violet-Visible spectrophotometer, modification of suede leather with silver nanoparticles, characterization of suede leather without modification with FTIR spectrophotometer, SEM observations, and antibacterial activity tests with diffusion techniques. The results showed that betel leaf extract could change silver nitrate salt to Ag nanoparticles as indicated by UV-Vis’ absorption which peaked at 259 nm and 456 nm. The FTIR spectrum showed that silver nanoparticles were successfully introduced into suede leather with the interaction between -OH free and -C = O free with Ag nanoparticles. SEM photos showed that Ag nanoparticles successfully layered the surface of suede leather tissue and the shape of the silver nanoparticles was spherical. Suede leather with the addition of silver nanoparticles had the best ability to cause the death of E. coli bacteria, outperforming leather without modification by 1.8 times and silver nanoparticles in incubation for 36 hours by 1.3 times. The incubation time significantly affected the inhibition in the growth of S. aureus bacteria by suede leather samples.