In targeted proteomics, the development of robust methodologies is dependent upon the selection of a set of optimal peptides for each protein-of-interest. Unfortunately, predicting which peptides and respective product ion transitions provide the greatest signal-to-noise ratio in a particular assay matrix is complicated. Using in vitro synthesized proteins as analytical standards, we report here an empirically driven method for the selection of said peptides in a human plasma assay matrix.
CITATION STYLE
Bollinger, J. G., Stergachis, A. B., Johnson, R. S., Egertson, J. D., & MacCoss, M. J. (2016). Selecting optimal peptides for targeted proteomic experiments in human plasma using in vitro synthesized proteins as analytical standards. Methods in Molecular Biology, 1410, 207–221. https://doi.org/10.1007/978-1-4939-3524-6_12
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