Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives

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Abstract

Wild-type phenylalanine dehydrogenase from Bacillus sphaericus, and three mutants N145A, N145V and N145L, are used with a coenzyme recycling system to synthesise l-phenylalanine and three non-natural amino acids (p-F-phenylalanine, p-MeO-phenylalanine and p-CF3-phenylalanine) on a millimole scale. A range of reaction conditions are investigated. The kinetic parameters of WT PheDH and N145A towards p-CF3-phenylpyruvate are compared, emphasising the value of protein engineering in creating improved biocatalysts. © 2006 Elsevier B.V. All rights reserved.

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Paradisi, F., Collins, S., Maguire, A. R., & Engel, P. C. (2007). Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives. Journal of Biotechnology, 128(2), 408–411. https://doi.org/10.1016/j.jbiotec.2006.08.008

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