Microalgal kinetics — a guideline for photobioreactor design and process development

Abstract

Kinetics generally describes bio-(chemical) reaction rates in dependence on substrate concentrations. Kinetics for microalgae is often adapted from heterotrophs and lacks mechanistic foundation, e.g. for light harvesting. Using and understanding kinetic equations as the representation of intracellular mechanisms is essential for reasonable comparisons and simulations of growth behavior. Summarizing growth kinetics in one equation does not yield reliable models. Piecewise linear or rational functions may mimic photosynthesis irradiance response curves, but fail to represent the mechanisms. Our modeling approach for photoautotrophic growth comprises physical and kinetic modules with mechanistic foundation extracted from the literature. Splitting the light submodel into the modules for light distribution, light absorption, and photosynthetic sugar production with independent parameters allows the transfer of kinetics between different reactor designs. The consecutive anabolism depends among others on nutrient concentrations. The nutrient uptake kinetics largely impacts carbon partitioning in the reviewed stoichiometry range of cellular constituents. Consecutive metabolic steps mask each other and demand a maximum value understandable as the minimum principle of growth. These fundamental modules need to be clearly distinguished, but may be modified or extended based on process conditions and progress in research. First, discussion of kinetics helps to understand the physiological situation, for which ranges of parameter values are given. Second, kinetics should be used for photobioreactor design, but also for gassing and nutrient optimization. Numerous examples are given for both aspects. Finally, measuring kinetics more comprehensively and precisely will help in improved process development.