Specific length amplified fragment sequencing (SLAF-seq) technology is a simplified genome sequencing technology based on next-generation sequencing. SLAF-seq technology has several distinguishing characteristics: 1. Deep sequencing to ensure accuracy of genotyping; 2. Effectively reduce sequencing costs; 3. Pre-designed simplified representation scheme to optimize marker efficiency; 4. Doubled barcode system for large populations. The advantages and technical process of SLAF-seq are described briefly with summarized results for the application of SLAF-seq in development of molecular markers, construction of high-density genetic map and gene mapping in ornamental plants. Finally, the difficulties and prospects of this method are discussed in application.
CITATION STYLE
Zhou, Y., & Pan, H. (2023). Specific-Locus Amplified Fragment Sequencing (SLAF-Seq). Methods in Molecular Biology (Clifton, N.J.), 2638, 165–171. https://doi.org/10.1007/978-1-0716-3024-2_11
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