Anti-Inflammatory and Cytotoxic Effect of Stevia and Neem Based Herbal Formulation

Abstract

Introduction: Nanotechnology is being used in developing countries to treat diseases and prevent health issues. Stevia, conjointly stated as Stevia rebaudiana member of the chrysanthemum family, a subgroup of the Asteraceae family. Azadirachta indica is a native tree to Asian nations. Azadirachta indica could facilitate the fight against plaque buildup and stop periodontal disease. Applied science in drugs needs the employment of nanoparticles to transmit the drug, heat, lightweight, or alternative substances to specific cell sorts like cancer cells. Aim: In this present investigation, Stevia and Neem-based plant extract was prepared and analyzed its anti-inflammatory and cytotoxic effects. Materials and Methods: The plant extract was prepared and an anti-inflammatory and cytotoxic effect was done using a UV-Beckmann spectrometer. The anti-inflammatory activity of nanoparticles prepared using plant extract was investigated by albumin denaturation assay. The results of the test were described as the standard deviation and analyzed using UV-Vis spectroscopy. For the cytotoxic activity, wells are used, wherein the mortality rate of the shrimps was estimated when the plant extract mediated of nanoparticles of different concentrations was added. Results: The nanoparticles synthesized using the stevia and neem extract showed the highest absorbance at a concentration of 50µL (90%) when subjected to inhibition of albumin denaturation assay to check for its anti-inflammatory activity. Brine shrimp lethality was done and the cytotoxicity of these nanoparticles was found to be increasing with increasing concentration of the administered nanoparticles. Conclusion: Anti-inflammatory and cytotoxic activity of Neem and Stevia mediated nanoparticles showed a successful outcome in both the assays.