Quantifying alterations in cell migration: Tracking fluorescently-tagged migrating cells by FACs and live-imaging

Abstract

Cell migration is fundamental to many different physiological processes including embryonic development, inflammation and wound healing. Given the range and importance cell migration plays a number of assays have been developed to measure different aspects of cell migration. Here we describe two different methods to analyze cell migration. The first method analyzes the migration of fluorescently tagged cells using Boyden chambers and FACs and the second looks at migration properties using time-lapse microscopy. © 2013 Springer Science+Business Media New York.